As governance takes on new forms and dimensions, it is rendered ever more complex and further removed from the people in whose name public power is exercised. This is a development that is particularly apparent in the multilayered and policentric construction of the EU, which does not follow a classic conception of separation of powers. We will analyse the emergence of new divisions of power in the EU against the traditional term referring to a designation and delimitation of public powers with mutual control structures and constraints between the three branches of government: legislative, executive, and judicial (Eckes, Leino-Sandberg, and Wallerman Ghavanini, 2021). This event is the concluding conference of the three-year research consortium ‘Separation of powers for 21st century Europe (SepaRope)’ between the Universities of Amsterdam, Gothenburg and Helsinki financed through the programme ‘NORFACE Democratic Governance in a Turbulent Age’ funded by a group of European Academies of Science and the European Commission through the Horizon2020 Programme.